Review Article
Microscopy-based Data Processing in Cell Biology
Issue:
Volume 13, Issue 1, June 2025
Pages:
1-22
Received:
16 May 2025
Accepted:
3 June 2025
Published:
23 June 2025
Abstract: By making it possible to extract intricate and significant biological information from visual imaging, data processing based on microscopy has completely changed contemporary cell biology. Researchers have overcome historical constraints by combining microscopy with sophisticated image processing technologies, opening up new possibilities for comprehending cellular architecture and functions in unprecedented detail. The goal of this study is to present a thorough examination of the methods and new developments in microscopy-driven data analysis, emphasizing both the theoretical underpinnings and real-world applications. The study starts by reviewing many microscopy techniques, including light, fluorescence, confocal, super-resolution, and electron microscopy, emphasizing their unique advantages and functions in contemporary cell biology. After that, it examines crucial picture preprocessing methods that are necessary for data dependability, such as contrast enhancement, background correction, and noise reduction. The segmentation and feature extraction techniques that allow precise cellular component detection and quantification are covered in detail. The article also describes new software and computational tools that facilitate automation and uniformity in the collection, processing, and analysis of images. The importance of quantitative analytic techniques for deciphering biological processes is also highlighted. These techniques include intensity measurement, colocalization, geographical distribution, and statistical analysis. Insights from microscope pictures are further improved by data visualization techniques like 3D rendering and machine learning software. The paper concludes by exploring emerging trends that have the potential to further change the field of microscopy in cell biology, including artificial intelligence, cloud-based platforms, multimodal imaging, and immersive technologies like augmented and virtual reality. To summarize, microscopy-based data processing is crucial to the advancement of cellular biology research, as it provides a wealth of opportunities for discovery through the integration of technology and multidisciplinary innovation.
Abstract: By making it possible to extract intricate and significant biological information from visual imaging, data processing based on microscopy has completely changed contemporary cell biology. Researchers have overcome historical constraints by combining microscopy with sophisticated image processing technologies, opening up new possibilities for compr...
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Research Article
Chemo-preventive Potential of Ethanolic Extract of Nigella sativum on N-nitroso Methyl Urea (NMU) Induced Breast Cancer in Female Albino Mice
Kehinde Sowunmi*,
Ogbonnaya Uchenna Nwoye,
Olayiwola Ibukun Esther,
Kade Emmanuel Ayomikun,
Ayobami Adesiyan Lawal
Issue:
Volume 13, Issue 1, June 2025
Pages:
23-29
Received:
10 May 2025
Accepted:
22 May 2025
Published:
26 August 2025
DOI:
10.11648/j.cb.20251301.12
Downloads:
Views:
Abstract: Nigella sativa is one of the most important medicinal plants in the realm of pharmaceuticals because of the outstanding healing potential of seeds. This is so because the plant offers numerous therapeutic benefits due to its diverse content of bioactive compounds, including alkaloids, carbohydrates, flavonoids, glycosides, phenols, resins, saponins, sterols, tannins, and terpenes. This work aimed at evaluating the chemo preventive effect of the ethanolic extract of N. sativa on NNMU induced breast cancer in female albino mice. Mature, female Albino mice but were weighed, and their average was 22. 5g each; 30 mice were divided into 4 equal groups. Group 1 animal was the control which was given distilled water. Group 3-5 animals received N. sativa ethanolic extract orally at the doses of 50mg/kg, 100mg/kg, and 200mg/kg body weight respectively, and Group 4, received only cisplatin. Blood, mammary glands and liver tissues were collected regarding hematologic, antioxidant biomarkers and biochemistry. The current plant extract treatments reduced NMU induced oxidative stress as indicated by enhanced levels of serum SOD in the treatment groups compared to the cisplatin group. Determination of total protein in the serum of treated mice showed and elevation in the total proteins compared to control group. A reduction was noted in the haemoglobin concentration, packed cell volume, red blood cell count, mean cell volume, and mean cell haemoglobin in all groups, which was significant at 50mg/kg N. sativa and cisplatin groups compared to the control. Percentage of neutrophil and lymphocyte level also showed significant difference compared with cisplatin and control group. These results indicate that the ethanolic extract of N. sativa leaves has chemo preventive properties and can be advocated as a prophylactic antidote against breast cancer.
Abstract: Nigella sativa is one of the most important medicinal plants in the realm of pharmaceuticals because of the outstanding healing potential of seeds. This is so because the plant offers numerous therapeutic benefits due to its diverse content of bioactive compounds, including alkaloids, carbohydrates, flavonoids, glycosides, phenols, resins, saponins...
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,
Rojina Khatun
