Volume 8, Issue 2, December 2020, Page: 33-38
Anti-wrinkle Effect of a Palmitoyl Oligopeptide Complex on Human Keratinocytes and Fibroblasts Through TGF-β1 Pathway
Yina Lu, Shanghai JAKA Biotech Co. Ltd, Shanghai, China
Guoxun Wang, Suzhou Greenleaf Daily Commodity Co., Ltd., Suzhou, China
Dong Liu, Suzhou Greenleaf Daily Commodity Co., Ltd., Suzhou, China
Jun Tian, Shanghai JAKA Biotech Co. Ltd, Shanghai, China
Received: Oct. 29, 2020;       Accepted: Nov. 9, 2020;       Published: Nov. 19, 2020
DOI: 10.11648/j.cb.20200802.13      View  15      Downloads  6
Abstract
Many researchers revealed that dermal fibroblast which mainly synthesis the collagen and elastin in dermis were affects by skin ageing, such as the proliferation rate and gene expression level. Oligopeptides were verified by many reports on anti-aging focus on collagen synthesis, but few literatures revealed the molecular mechanism of oligopeptides on skin aging. The potential anti-ageing effect and the mechanism of PTC, a new palmitoyl oligopeptide complex, were evaluated. MTT assay was adopted to detect the anti-aging effect of PTC in both human dermal fibroblast HDF-a and keratinocyte NHEK. The mechanism of PTC was detected further by full transcriptome analysis using RNA-sequencing method, and ELISA analysis of key proteins. Skin wrinkles were measured by VC98 in vivo. PTC showed positive effect in cell proliferation and the biosynthesis of collagens, intergrins not only on fibroblasts, but also on keratinocytes. Moreover, many pathways were confirmed in the anti-aging effect of PTC, such as TGF-β1 pathway and ECM-receptor interaction. A cream contained 5.0% PTC showed significant improvement of skin wrinkles and roughness. So that, PTC has a potentially beneficial effect on the cell proliferation, the biosynthesis of ECM related proteins and the expression of many genes which are involved in cell cycle and secretory features to slow down skin aging. The molecular pathway maybe targets the TGF-β1 in both epidermis and dermis.
Keywords
Anti-wrinkle, A Palmitoyl Oligopeptide Complex, Transcriptome, TGF-β1
To cite this article
Yina Lu, Guoxun Wang, Dong Liu, Jun Tian, Anti-wrinkle Effect of a Palmitoyl Oligopeptide Complex on Human Keratinocytes and Fibroblasts Through TGF-β1 Pathway, Cell Biology. Vol. 8, No. 2, 2020, pp. 33-38. doi: 10.11648/j.cb.20200802.13
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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