Volume 8, Issue 1, June 2020, Page: 8-11
Culture of Human Gingival Fibroblasts: An Experimental Model
Raoul Bationo, CHU de Bogodogo, Ouagadougou, Burkina Faso, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire
Ablassé Rouamba, Université Joseph Ki-Zerbo/Laboratoire de Biochimie et de Chimie Appliquées, Ouagadougou, Burkina Faso
Abdoulaziz Diarra, CHU de Tengandogo, Université Joseph Ki-Zerbo/UFR SDS, Ouagadougou, Burkina Faso
Monique Lydie Beugré-Kouassi, Département d’Orthopédie Dento-Faciale/UFR d’Odonto-stomatologie, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire
Fabienne Jordana, CHU de Nantes/Service d’Odontologie, Université de Nantes/UFR d’Odontologie, Nantes, France
Jean-Bertin Beugré, Département d’Orthopédie Dento-Faciale/UFR d’Odonto-stomatologie, Université Félix Houphouët-Boigny, Abidjan, Côte d’Ivoire
Received: May 5, 2020;       Accepted: Jun. 11, 2020;       Published: Jun. 20, 2020
DOI: 10.11648/j.cb.20200801.12      View  160      Downloads  73
Abstract
Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. It is used as a means for in vitro testing of the biocompatibility of resin polymers used in dentistry. The majority of cells are cultured with Dulbecco's modified Eagle's medium (DMEM) or RPMI supplemented with fetal bovine serum. Several cell types are being studied including gingival fibroblasts. Gingival fibroblasts are the main cells of gingival connective tissue. These cells play an active and important role in almost all coating fabric processes, and its involvement in various pathophysiological conditions, including, healing, repair, aging, psoriasis, cancer among others, is only beginning to be understood. DMEM is the most widely used fibroblastic culture medium. This model describes a method for obtaining and cultivating human gingival fibroblasts, by explants derived from surgical discards. Fibroblasts were isolated mechanically and cultured in RPMI 1640 culture medium supplemented with fetal bovine serum 10%, Penicillin (10000 U/ml)/Streptomycin (10 mg/ml) 1% and L-Glutamine (200 mM) 1%. The culture medium is replaced every two days. Cells forming a fairly dense network were observed after a period of 4 days of culture. Human gingival fibroblasts can be cultured by direct explant technique with RPMI 1640 culture medium supplemented with fetal bovine serum and antibiotics.
Keywords
Cell Culture, Gingival Fibroblast, RPMI 1640 Culture Medium
To cite this article
Raoul Bationo, Ablassé Rouamba, Abdoulaziz Diarra, Monique Lydie Beugré-Kouassi, Fabienne Jordana, Jean-Bertin Beugré, Culture of Human Gingival Fibroblasts: An Experimental Model, Cell Biology. Vol. 8, No. 1, 2020, pp. 8-11. doi: 10.11648/j.cb.20200801.12
Copyright
Copyright © 2020 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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