Volume 6, Issue 1, June 2018, Page: 13-19
Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation
Shuhei Soeda, Laboratory of Neurochemistry, College of Pharmaceutical Sciences, Ritsumeikan University, Shiga, Japan
Hideo Taniura, Laboratory of Neurochemistry, College of Pharmaceutical Sciences, Ritsumeikan University, Shiga, Japan
Received: Jun. 3, 2018;       Accepted: Jul. 5, 2018;       Published: Aug. 2, 2018
DOI: 10.11648/j.cb.20180601.13      View  1418      Downloads  177
Sirtuin is an evolutionally conserved histone deacetylase. The effects of sirtuin activator, resveratrol and inhibitor, nicotinamide on early Dictyostelium development upon starvation were examined. The cell streaming was appeared at 7 h and tight aggregates was formed at 24 h in control cells under submerged culture. Resveratrol treatment accelerated the development. Cell streaming at 6 h and weak aggregates at 9 h were already observed. While, nicotinamide treatment delayed the development. The initiation of streaming was delayed at 9 h, and the development was still under a cell streaming even after 24 h. Dictyostelium development starts inducing the essential molecules adenylyl cyclase, aca and cAMP pulsing induces cell-surface cAMP receptor (carA). Resveratrol treatment significantly increased the expression level of aca at 4 and 6 h after starvation. Nicotinamide treatment decreased the aca expression level at 4 h and carA expression level at 6 h. The induction of aca upon starvation starts with the transcriptional activation by MybB, a. putative transcription factor. MybB expression recovered the initiation timing of the cell streaming against the effect of nicotinamide, but the aggregates formation was still impaired at 24 h. Resveratrol had no effects on MybB-null mutant cells. Neither streaming nor aggregates formation was observed even after 24 h with low levels of aca expression after starvation by resveratrol treatment. These findings suggest that sirtuins may participate in more than one point of the early developmental processes of Dictyostelium upon starvation.
Sirtuin, Resveratrol, Nicotinamide, Cellular Development, Dictyostelium discoideum
To cite this article
Shuhei Soeda, Hideo Taniura, Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation, Cell Biology. Vol. 6, No. 1, 2018, pp. 13-19. doi: 10.11648/j.cb.20180601.13
Copyright © 2018 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
S. Imai, C. M. Armstrong, M. Kaeberlein, and L. Guarente. “Transcriptional silencing and longevity protein Sir2 is an NAD-dependent histone deacetylase,” 2000, Nature 403, 795-800.
J. Landry, A. Sutton, S. T. Tafrov, R. C. Heller, J. Stebbins, L. Pillus, and R. Sternglanz. “The silencing protein SIR2 and its homologs are NAD-dependent protein deacetylases,” 2000, Proc. Natl. Acad. Sci. USA 97, 5807-5811.
H. Yamamoto, K. Schoonjans, and J. Auwerx. “Sirtuin functions in health and disease,” Mol. Endoclinol. 2007, 21, 1745-1755.
S. Michan, and D. Sinclair. “Sirtuins in mammals: insights into their biological function,” 2007, Biochem. J. 404, 1-13.
P. Schaap. “Evolution of developmental signaling in Dictyostelid social amoebas,” 2016, Curr. Opin. Genet. Dev. 39, 29-34.
H. Otsuka, and P. J. M. Van Haastert. “A novel Myb homolog initiates Dictyostelium development by induction of adenylyl cyclase expression,” 1998, Genes Develop. 12, 1738-1748.
J. Rodriguez-Centeno, and L. Sastre. “Biological activity of the alternative promoters of the Dictyostelium discoideum adenylyl cyclase A gene,” 2016, PLOS ONE 0148533.
T. Katayama, and H. Yasukawa. “Analysis of Sir2E in the cellular slime mold Dictyostelium discoideum: Cellular localization, spatial expression and overexpression,” 2008, Develop. Growth. Differ. 50, 645-652.
F. Picard, M. Kurtev, N. Chung, A. Topark-Ngram, T. Senewong, R. M. de Oliveira, M. Leid, M. W. McBurney, and L. Guarente. “Sirt1 promotes fat mobilization in white adipocytes by repressing PPAR-g,” 2004, Nature 429, 771-777.
A. Brunet, L. B. Sweeney, J. F. Sturgill, K. F. Chua, P. L. Greer, Y. Lin, H. Tran, S. E. Ross, R. Mostoslavsky, H. Y. Cohen, L. S. Hu, H-L. Cheng, M. P. Jedrychowski, S. P. Gygi, D. A. Sinclair, F. W. Alt, and M. E. Greenberg. “Stress-dependent regulation of FOXO transcription factor by the SIRT1 deacetylase,” 2004, Science 303, 2011-2015.
S. Nemoto, M. M. Fergusson, and T. Finkel. “SIRT1 functionally interacts with metabolic regulator and transcriptional coactivator PGC-1a, 2005, J. Biol. Chem. 280, 16456-16460.
H. Taniura, N. Tanabe, Y. Bando, and N. Arai. “Nse1 and Nse4, subunits of the Smc5-Smc6 complex, are involved in Dictyostelium development upon starvation,” 2015, Develop. Growth. Differ. 57, 430-443.
A. Vassilopoulos, K. S. Fritz, D. R. Petersen, and D. Gius. “The human sirtuin family: Evolutionary divergences and functions,” 2011, Human Genomics 5, 485-496.
T. J. Sun, and P. N. Devreotes. “Gene targeting of the aggregation stage cAMP receptor cAR1 in Dictyostelium,” 1991, Genes Develop. 5, 572-582.
X. Mu, S. A. Spanos, J. Shiloach, and A. Kimmel. “CRTF is a novel transcription factor that regulate multiple stages of Dictyostelium development.” 2001, Development 128, 2569-2579.
T. Kon, H. Adachi, and K. Sutoh. “amiB, a novel gene required for the growth/differentiation transition in Dictyostelium,” 2000, Genes to Cells 5, 43-55.
Browse journals by subject